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Morphological changes of UG-LIKE structures in collagen I-supplemented Matrigel, with or without the allosteric collagen <t>I-binding</t> <t>integrin</t> inhibitor and <t>ROCK</t> inhibitor. Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0, 0.1, or 0.5 mg/ml bovine collagen I ( N = 5, mean ± SEM). Different superscripts indicate significant differences among collagen I concentrations, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (A). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, with or without 0, 10, or 50 μM allosteric collagen I-binding integrin inhibitor (TC-l 15) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, combining collagen I supplementation and TC-l 15 treatment as a single factor, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (B). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, in the presence or absence of 0 or 10 μM ROCK inhibitor (Y-27632) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, with collagen I supplementation and Y-27632 treatment as two independent factors, as determined by two-way ANOVA of logit-transformed data (Supplementary Table S4, P < 0.05). Scale bars represent 1000 μm (C).
Rock Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Morphological changes of UG-LIKE structures in collagen I-supplemented Matrigel, with or without the allosteric collagen <t>I-binding</t> <t>integrin</t> inhibitor and <t>ROCK</t> inhibitor. Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0, 0.1, or 0.5 mg/ml bovine collagen I ( N = 5, mean ± SEM). Different superscripts indicate significant differences among collagen I concentrations, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (A). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, with or without 0, 10, or 50 μM allosteric collagen I-binding integrin inhibitor (TC-l 15) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, combining collagen I supplementation and TC-l 15 treatment as a single factor, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (B). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, in the presence or absence of 0 or 10 μM ROCK inhibitor (Y-27632) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, with collagen I supplementation and Y-27632 treatment as two independent factors, as determined by two-way ANOVA of logit-transformed data (Supplementary Table S4, P < 0.05). Scale bars represent 1000 μm (C).
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Morphological changes of UG-LIKE structures in collagen I-supplemented Matrigel, with or without the allosteric collagen <t>I-binding</t> <t>integrin</t> inhibitor and <t>ROCK</t> inhibitor. Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0, 0.1, or 0.5 mg/ml bovine collagen I ( N = 5, mean ± SEM). Different superscripts indicate significant differences among collagen I concentrations, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (A). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, with or without 0, 10, or 50 μM allosteric collagen I-binding integrin inhibitor (TC-l 15) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, combining collagen I supplementation and TC-l 15 treatment as a single factor, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (B). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, in the presence or absence of 0 or 10 μM ROCK inhibitor (Y-27632) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, with collagen I supplementation and Y-27632 treatment as two independent factors, as determined by two-way ANOVA of logit-transformed data (Supplementary Table S4, P < 0.05). Scale bars represent 1000 μm (C).
Rock Inhibitor, supplied by Biogen Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Morphological changes of UG-LIKE structures in collagen I-supplemented Matrigel, with or without the allosteric collagen <t>I-binding</t> <t>integrin</t> inhibitor and <t>ROCK</t> inhibitor. Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0, 0.1, or 0.5 mg/ml bovine collagen I ( N = 5, mean ± SEM). Different superscripts indicate significant differences among collagen I concentrations, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (A). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, with or without 0, 10, or 50 μM allosteric collagen I-binding integrin inhibitor (TC-l 15) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, combining collagen I supplementation and TC-l 15 treatment as a single factor, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (B). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, in the presence or absence of 0 or 10 μM ROCK inhibitor (Y-27632) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, with collagen I supplementation and Y-27632 treatment as two independent factors, as determined by two-way ANOVA of logit-transformed data (Supplementary Table S4, P < 0.05). Scale bars represent 1000 μm (C).
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Morphological changes of UG-LIKE structures in collagen I-supplemented Matrigel, with or without the allosteric collagen <t>I-binding</t> <t>integrin</t> inhibitor and <t>ROCK</t> inhibitor. Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0, 0.1, or 0.5 mg/ml bovine collagen I ( N = 5, mean ± SEM). Different superscripts indicate significant differences among collagen I concentrations, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (A). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, with or without 0, 10, or 50 μM allosteric collagen I-binding integrin inhibitor (TC-l 15) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, combining collagen I supplementation and TC-l 15 treatment as a single factor, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (B). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, in the presence or absence of 0 or 10 μM ROCK inhibitor (Y-27632) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, with collagen I supplementation and Y-27632 treatment as two independent factors, as determined by two-way ANOVA of logit-transformed data (Supplementary Table S4, P < 0.05). Scale bars represent 1000 μm (C).
Y 27632 Rock Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Morphological changes of UG-LIKE structures in collagen I-supplemented Matrigel, with or without the allosteric collagen <t>I-binding</t> <t>integrin</t> inhibitor and <t>ROCK</t> inhibitor. Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0, 0.1, or 0.5 mg/ml bovine collagen I ( N = 5, mean ± SEM). Different superscripts indicate significant differences among collagen I concentrations, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (A). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, with or without 0, 10, or 50 μM allosteric collagen I-binding integrin inhibitor (TC-l 15) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, combining collagen I supplementation and TC-l 15 treatment as a single factor, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (B). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, in the presence or absence of 0 or 10 μM ROCK inhibitor (Y-27632) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, with collagen I supplementation and Y-27632 treatment as two independent factors, as determined by two-way ANOVA of logit-transformed data (Supplementary Table S4, P < 0.05). Scale bars represent 1000 μm (C).
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Morphological changes of UG-LIKE structures in collagen I-supplemented Matrigel, with or without the allosteric collagen I-binding integrin inhibitor and ROCK inhibitor. Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0, 0.1, or 0.5 mg/ml bovine collagen I ( N = 5, mean ± SEM). Different superscripts indicate significant differences among collagen I concentrations, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (A). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, with or without 0, 10, or 50 μM allosteric collagen I-binding integrin inhibitor (TC-l 15) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, combining collagen I supplementation and TC-l 15 treatment as a single factor, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (B). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, in the presence or absence of 0 or 10 μM ROCK inhibitor (Y-27632) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, with collagen I supplementation and Y-27632 treatment as two independent factors, as determined by two-way ANOVA of logit-transformed data (Supplementary Table S4, P < 0.05). Scale bars represent 1000 μm (C).

Journal: Bioscience Reports

Article Title: Tubulogenesis of bovine uterine glands by epidermal growth factor and collagen I in 3D culture systems

doi: 10.1042/BSR20260010

Figure Lengend Snippet: Morphological changes of UG-LIKE structures in collagen I-supplemented Matrigel, with or without the allosteric collagen I-binding integrin inhibitor and ROCK inhibitor. Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0, 0.1, or 0.5 mg/ml bovine collagen I ( N = 5, mean ± SEM). Different superscripts indicate significant differences among collagen I concentrations, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (A). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, with or without 0, 10, or 50 μM allosteric collagen I-binding integrin inhibitor (TC-l 15) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, combining collagen I supplementation and TC-l 15 treatment as a single factor, as determined by one-way ANOVA of logit-transformed data ( P < 0.05). Scale bars represent 1000 μm (B). Lengths of UG-LIKE structures on day 5 in Matrigel supplemented with 0 or 0.5 mg/ml bovine collagen I, in the presence or absence of 0 or 10 μM ROCK inhibitor (Y-27632) ( N = 5, mean ± SEM). Different superscripts indicate significant differences among experimental groups, with collagen I supplementation and Y-27632 treatment as two independent factors, as determined by two-way ANOVA of logit-transformed data (Supplementary Table S4, P < 0.05). Scale bars represent 1000 μm (C).

Article Snippet: To further assess how collagen I influences the morphological change of 3D-cultured uterine gland fragments, a potent α1β1 and α2β1 integrin inhibitor (TC-l 15; HY-107588, MedChemExpress LLC, NJ, USA) or ROCK inhibitor (Y-27632; HY-10583, MedChemExpress LLC) was added to the culture media.

Techniques: Binding Assay, Transformation Assay